Abstract
Abstract Shoots forced from dormant stems and nodal explants from current season’s growth of a 24-year-old sourwood [Oxydendrum arboreum (L.) DC] were cultured on Woody Plant Medium (WPM) supplemented with zeatin. The greatest number of shoots >10 mm was produced with 4 μM zeatin, whereas BA and 2iP were ineffective at the same concentrations. Addition of IAA (5 and 10 μM) to the medium reduced the number of shoots. Thidiazuron (0.0045–0.045 μM) produced a few short shoots. In vitro rooting of the proliferated shoots was enhanced with 2.5 to 10 μM of IBA in the culture medium. Chemical names used: N-(phenylmethyl)-lH-purin-6-amine (BA); 1H-indole-3-acetic acid (IAA); 1H-indole-3-butyric acid (IBA); N-(3-methyl-2-butenyl)-1H-purin-6-amine (2iP); 1-naphthaleneacetic acid (NAA); 2-methyl-4-(1H-purin-6-ylamino)-2-niten-1-ol (zeatin); N-phenyl-N’-1,2,3-thiadiazol-5-ylurea (thidiazuron, TDZ).
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