Abstract
A protocol for in vitro clonal propagation of the valuable medicinal plant— Pueraria lobata by enhanced axillary bud proliferation in shoot tip explants is described. MS medium supplemented with 4.6 μM KIN and 5.7 μM IAA induced an optimum frequency of shoot formation (94%) and shoot number (3.6 shoots per explant). Subculture of shoot tip explants harvested from in vitro-derived shoots on the multiplication medium enabled continuous shoot production with a similar frequency. The best shoot elongation was achieved on the MS medium containing 5.8 μM GA 3 and 4.4 μM BA. Rooting was the highest (100%) on the full-strength MS medium with 2.68 μM NAA or 2.85 μM or 11.42 μM IAA. Micropropagated plantlets were successfully hardened to survive ex vitro conditions and then established into the soil. TLC and preliminary quantitative HPLC analysis indicated that micropropagated plants and callus tissue produced the main isoflavonoid compounds as those present in the leaves and roots of intact plants of P. lobata.
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