In Vitro Propagation of Hosta plantaginea1

Abstract

Abstract Callus was initiated from cultured immature inflorescences of Hosta plantaginea Asch. ‘Grandiflora’ in darkness on a basal medium containing Murashige and Skoog salts, vitamins, glycine, i-inositol, sucrose, agar, and 10 mg/liter naphthaleneacetic acid (NAA) and 0.1 mg/liter 6-benzylamino purine (BA). Reculture of the callus onto a medium containing 0.01 mg/liter NAA and 5 mg/liter BA, resulted in the formation of numerous adventitious shoots. Adventitious shoots were initiated also from leaves and segments of leaves from shoots formed in vitro when cultured on a medium supplemented with 0.01-1 mg/liter NAA and 5 mg/liter BA. Root formation was achieved when individual shoots were excised and transferred to a medium containing the basal constituents without growth regulator supplements. Rooted plants were successfully transferred to soil.