In vitro propagation of Drosera intermedia as influenced by cytokinins, pH, sucrose, and nutrient concentration

Jindrich Rejthar,Petra Cepkova,Iva Viehmannova,Luigi Milella,Eloy Fernndez

doi:10.9755/ejfa.v26i6.18022

Jindrich Rejthar, Petra Cepkova + Show 3 more

Open Access

https://doi.org/10.9755/ejfa.v26i6.18022

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Abstract

This study was aimed to optimize in vitro propagation of Drosera intermedia for commercial and conservation purposes. The effect of concentration of MS nutrients (⅛ MS, ¼ MS, ½ MS and, MS), various pH (3.7-7.7), sucrose concentration (10-40 g l -1 ) and cytokinins (0.1-3 mg l -1 ), namely BA (N6-benzyladenine), kinetin and zeatin were evaluated. After 60 days of shoot cultivation, growth and developmental characteristics (plant height, number of shoots per explant, diameter of rosette, number of roots per explant, length of roots) were recorded. No significant differences were found for various levels of pH and sucrose. On the contrary, plant height was negatively influenced by an increase of nutrients in the medium. The plants on 1/8 MS medium were significantly taller, and displayed higher proliferation capacity compared with those cultivated on full-strength MS medium. Shoot multiplication and growth was suppressed by supplementation of BA and kinetin, regardless of concentration used. Zeatin at the lowest concentration (0.1 mg l -1 ) provided the best results for shoot proliferation of all 26 treatments and can be recommended for micropropagation of D. intermedia.

Highlights

The Drosera genus (Droseraceae) consists of carnivorous plants with active flypaper traps and includes nearly 150 species distributed in Australia, Africa, and South America, with some Northern Hemisphere species (Rivadavia et al, 2003)
D. intermedia had been used for artificial hybridization with D. anglica and D. capillaris (Kusakebe, 1979)
Multiplication The results revealed that plants cultivated on medium with lower concentrations of sucrose (10 or 20 g l-1) show higher proliferation capacity and had a higher diameter of rosettes, the differences among these characteristics were not statistically significant when compared to other treatments

Summary

Introduction

The Drosera genus (Droseraceae) consists of carnivorous plants with active flypaper traps and includes nearly 150 species distributed in Australia, Africa, and South America, with some Northern Hemisphere species (Rivadavia et al, 2003). Several bioactive compounds from sundew leaves and roots including flavonoids and quinones have been found (Hook, 2001; Marczak et al, 2005; Putalun et al, 2010). These compounds can be considered as very important metabolites (Milella et al, 2011; Padula et al, 2013). D. intermedia is an attractive species of potential horticultural value. It is sized between D. anglica and D. rotundifolia, bearing wedge-shaped leaves, 7-12 mm long, and 4-10 mm wide, in a rosette. A spontaneous hybrid D. intermedia x D. capillaris, interspread between colonies of the two species, was discovered (Sheridan, 1987)

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