In vitro propagation of Byblis liniflora Salisb

Abstract

This study was conducted to develop an in vitro propagation procedure for Byblis liniflora Salisb. The process of seed sterilization and in vitro sowing achieved 95% efficiency. From the material aseptic stem segments, the shoot proliferation program was determined with the rate of 33.67 shoots/explants after 4 weeks of culture on nutrient medium (1/3 strength MS basal salts plus full Morel and Wetmore’s vitamins) with addition of a mixture of cytokinin (0.5 mg/L Kin and 2 mg/L BA) and then transferred to culture for another 4 weeks on nutrient medium. The rooting program from aseptic stem segments was to culture in nutrient medium supplemented with 0.5 mg/L NAA for 8 weeks. To produce a complete plant, single shoot separated from the proliferative clusters was transferred to a culture flask containing nutrient medium and cultured for 4 weeks. The in vitro shoot developed more branches and self-rooted to form a complete plant. The suitable growing substrate in the acclimatization and outdoor stages was a mixture of peat moss and perlite (1 : 2 w/w). The climatic conditions of Ho Chi Minh City and the Southeast of Vietnam were suitable for growing Byblis liniflora trees outdoors all year round.