Abstract
Abstract An in vitro method of propagating Begonia x tuberhybrida Voss from leaf sections is described. Leaf sections (2 x 2 cm), including a major vein, are submerged partially in a semisolid, bud-inducing medium, containing Murashige and Skoog (MS) salts, vitamins, sucrose, 1.0 mg/liter naphthalene-acetic acid (NAA), and 5.0 mg/liter 6-benzylamino purine (BA). Cultures are maintained in a 16-hr photoperiod at 20°C. After 8 to 10 weeks, the explants, which have begun bud differentiation, are divided and subcultured to a liquid medium containing MS salts, vitamins, sucrose, 1 mg/liter NAA and 1 mg/liter BA, to induce proliferation and development of propagules. The propagules then are placed, in vivo, in an indolebutyric acid (IBA) solution for 10 days to induce rooting. The total time elapsed between taking explants from leaves and the transfer of rooted propagules to pots is just over 5 months.
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