In vitro propagation of Bambusa balcooa by plant tissue culture technique

Abstract

Bambusa balcooa is a common plant grown in Bihar, Orissa, Jharkhand and Uttarakhand states of India having good cultural importance. The present experimentation on nodal explants of B. balcooa on MS media supplement under particular culture conditions has shown shunted growth in 30 days. Shoot tip explants of B. balcooa seedlings produced multiple shoots on MS medium supplemented with different plant growth regulators (PGRs) individually and in combination. Shoot tip explants of B. balcooa requires 30 days to initiate shoots. Among the three cytokinins tested, BAP was selected as the most suitable hormone to induce shoot multiplication. The highest shoot multiplication is found by incorporation with BAP (1.5 µg/L and 2 µg/L). The shoot multiplication rates are good with shoot length 2.5±0.3 cm and 3.9±0.4 cm with the highest and maximum shoot generation. The shoot multiplication rate is moderate with a shoot length of 2.00±0.5 cm. Multiplication potentiality was observed in the cluster having more than 2–3 shoots. The best period for recycling multiplying shoots is 2–3 weeks in the old culture. Delaying of the sub-culturing period resulted in gradual browning of the shoots. The sub-culturing period was recorded as the most crucial factor for obtaining the optimal and desired level of regeneration of shoots. The well-grown propagules in the present experimentation were shown successful regeneration. Hence, the experiment concluded with the best growth pattern observed in media containing auxins like BAP and cytokinins like kinetin.