In Vitro Propagation of Anigozanthos manglesii, Anigozanthos flavidus, and Macropidia fuliginosa1

Abstract

Abstract Rapid in vitro propagation of Anigozanthos manglesii D. Don. was achieved through the initiation and proliferation of shoots from lateral bud explants using a modified Murashige and Skoog medium, or Gamborg, Miller and Ojima B5 medium, supplemented with 0.5 ppm benzylaminopurine (BA) and 0.5 ppm naphthaleneacetic acid (NAA). Continued proliferation was obtained by sub-culture either on the same media or media containing 0.25 ppm BA plus 0.25 ppm NAA. Rapid rooting of shoots occured following transfer to a half strength B5 medium containing 0.2 ppm NAA. Plantlets were successfully transplanted onto a University of California (UC) potting mix under mist and reduced light intensity. The method has also been successfully employed with Anigozanthos flavidus Redoute and with Macropidia fulginosa (Hook.) Druce.