In vitro propagation of a useful tropical bamboo, Thyrsostachys siamensis Gamble, through shoot-derived callus

Abstract

We established a protocol for mass propagation of Thyrsostachys siamensis Gamble in vitro culture via callus formation. Single node cuttings were placed in Murashige and Skoog (MS) medium with benzyl adenine (BA) concentrations ranging from 0 to 44.40 μM. Although multiple shoots formed in all BA treatments, optimum shoot formation occurred at 11.10 μM BA. The MS medium for optimum callus induction from the multiple shoots contained 11.3 μM of 2,4-dichlorophenoxyacetic acid (2,4-D), 4.65 μM kinetin, and 1.96 μM indole-3-yl-butyric acid (IBA). The highest number of shoots regenerating from the callus was obtained in MS medium containing 11.1 μM N6-BA and 3.43 μM IBA. Shoot clusters of 3–5 shoots were rooted on MS medium supplemented with 26.85 μM 1-naphthaleneacetic acid for 3 weeks, and were then transferred onto MS medium without plant growth regulators for 1 week. This protocol can be used for gene manipulation in a breeding program, germplasm preservation, and mass propagation of the bamboo.