Abstract
Clonal propagation and multiple shoot formation in pistachio (Pistacia vera cv. Kirmizi) has been achieved with cotyledonary nodes devoid of cotyledons. Shoot development was induced on MS medium supplemented with 0.44-88 μM benzyladenine (BA) or kinetin. Both type and concentration of cytokinins significantly influenced shoot proliferation. Incorporation of gibberelic acid (0.2-1.0 μM) or naphthaleneacetic acid (0.5-5.3 μM) in the medium supplemented with cytokinins also effected the proliferation success. MS medium supplemented with 88 μM BA induced up to 5 shoots per explant with an average shoot length of 8.50 mm in 30 days. Further multiplication of shoots required continued culturing up to three subcultures of 30 days each on the same medium after establishment of cultures. Shoots formed in vitro were rooted on half-strength MS basal medium supplemented with 12.3 μM indole-3-butyric acid. Plantlets were successfully acclimated and established in soil.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
