In vitro Propagation and Genetic Assessment by using Markers in Potato (Solanum tuberosum L.) cv Kufri Ganga

Abstract

The purpose of this study was to determine the best types and concentrations of plant growth regulators to use during different stages of micropropagation, as well as to assess the genetic fidelity of tissue culture raised plants of a cv Kufri Ganga using reliable markers. According to the data on shoot bud initiation, a combination of 0.25 mgl-1 BAP and 0.5 mgl-1 Kinetin results in the greatest number of shoots per explant (3.4±0.11) in the shortest amount of time (3.2±0.09) days. The maximum number of in vitro shoots per shootlet (4.7±0.15) was recorded when auxins were combined with cytokinin (MS medium + 0.25 mgl-1 BAP + 0.01 mgl-1 IAA) for shoot proliferation. Root initiation was observed in (2.2±0.10) days on MS medium fortified with 1.5 mgl-1 NAA. The maximum number of in vitro roots per shoot (14.2±0.31) was observed when MS media was fortified with 2.0 mgl-1 IBA. Molecular markers were used to assess genetic fidelity in plants grown in vitro. Monomorphic DNA banding patterns were found in all tissue culture raised plants and mother plants, indicating true to type planting material. This protocol for tissue culture propagation, as well as testing its genetic fidelity, may be beneficial for the successful application of better conservation of germplasm.