In vitro propagation and conservation of rare species Fritillaria meleagris L. from floral explants

Abstract

The peculiarities of the in vitro regeneration of the rare species Fritillaria meleagris L. from floral explants have been studied for the first time. Upon the establishment of in vitro culture and during the multiplication stage, the most effective is the B5 nutrient medium supplemented with 0.44 µM BAP, 3.22 µM NAA, and 2.28 µM IAA. During the multiplication stage, the regeneration rate reaches 80% and the number of bulblets per explant is 3.9 ± 0.3. It is established that the morphogenesis of de novo formed structures depends on the mineral composition of the medium: the use of mineral-based B5 leads to adventitious shoot formation (gemmogenesis), while the use of BDS causes morphogenic callus formation and the gemmorhizogenesis on its surface. The stimulating effect of low temperatures (+7°C) on bulblet development and adaptation to ex vitro conditions is noted.