﻿In vitro production of flavonoids in cultures of Gypsophila glomerata

Pavlinka Popova,Iliana Ionkova,Yancho Zarev,Aleksandar Shkondrov,Ilina Krasteva

doi:10.3897/pharmacia.69.e77769

Abstract

Effects of increased concentration of calcium chloride on growth and production of flavonoids in newly established shoot and callus Gypsophila glomerata cultures were studied. The highest impact of CaCl2 on the growth index was determined in callus cultures (GI = 0.92), while in shoot cultures calcium treatment reduced the amount of biomass (GI = 0.38). Total flavonoids in shoot cultures grown on MS medium and MS medium supplemented with double amount of CaCl2 were 0.36 mg/g d. w. In both callus cultures, 2 mg/g d. w. total flavonoids were quantified. Shoots and callus grown on non-modified media accumulated 0.02 mg/g d. w. quercetin derivatives. Unlike these, both shoots and callus grown on calcium-enriched media accumulated 0.03 and 0.05 mg/g d. w. of isorhamnetin derivatives. In vitro shoot cultures grown on MS medium enriched in twice the amount of CaCl2 accumulated the highest amount of saponarin (0.138 mg/mg d. w.).

Highlights

The genus Gypsophila L. (Caryophyllaceae) comprises about 150 species of annual to perennial herbaceous plants distributed in the temperate regions of Asia, Europe, Africa, the Pacific Islands, and Australia
Growth index was detected in shoots, grown on MS medium supplemented with double amount of CaCl2
Shoots grown on MS and MS supplemented with an additional amount of CaCl2 had a similar quantity of total flavonoids – 0.36 mg/mg d. w. and alternatively, calli both had nearly 2 mg/ mg d. w

Summary

Introduction

The genus Gypsophila L. (Caryophyllaceae) comprises about 150 species of annual to perennial herbaceous plants distributed in the temperate regions of Asia, Europe, Africa, the Pacific Islands, and Australia. (Caryophyllaceae) comprises about 150 species of annual to perennial herbaceous plants distributed in the temperate regions of Asia, Europe, Africa, the Pacific Islands, and Australia. The plants are found in calcium-rich places that are high in gypsum, the name of the genus. In the Bulgarian flora, the genus Gypsophila is represented by six species: G. paniculata L., G. muralis L., G. glomerata Pall. Bieb., G. trichotoma Wend., G. tekyra Stef. G. petraea (Baumg.) Reichenb (Valev 1966). Gypsophila species are known for their industrial, decorative, and medical applications. Some species are widely used in traditional medicine, such as expectorants, diuretics, contraceptives, for the treatment of hepatitis, gastritis, and bronchitis (Frechet et al 1991; Jia et al 2002; Yücekutlu and Bildacı 2008; Yao et al 2010)

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Conclusion