In vitro production of double haploid plants from two rice species (Oryza sativa L. and Oryza glaberrima Steudt.) for the rapid development of new breeding material

Abstract

Eight genotypes of two rice species (Oryza sativa and Oryza glaberrima) were studied for their response to anther culture in terms of callus induction and frequency of plant regeneration. N6 medium (Chu et al., 1975) was used for callus induction, and MS medium (Murashige and Skoog, 1962) with 1 mg/l BAP and 0.5 mg/l NAA for plant regeneration. Generally, O. glaberrima genotypes produced more callus than O. sativa genotypes. All O. glaberrima genotypes regenerated plants. Among O. sativa genotypes only japonica variety IKP produced plants. Other O. sativa genotypes showed low frequency of callus induction without plant regeneration. Many albino plants were obtained from the culture. Only one O. glaberrima genotype (6202 Tog) produced green plants. In this experiment, a total of 93 plants were regenerated with 14 green plants and 79 albino plants. Anther culture response is largely species and genotype dependent. Key words: Rice species, Oryza sativa (L.), Oryza glaberrima (Steudt.), anther culture, medium, callus induction, plant regeneration.