Abstract
The aim of this research was to identify candidate probiotic lactic bacteria among indigenous dadih lactic isolates. Dadih is an Indonesian traditional fermented milk of West Sumatra which is fermented naturally. Viability of the strain is critical in determining the capacity of lactic bacteria to induce immune stimulation as well as to colonize in the intestinal tract. Therefore, LAB are proposed to exert health promoting or probiotic effects in human, such as inhibition of pathogenic microflora, antimutagenic, and the reduction of cholesterol levels. This manuscript reports in vitro probiotic properties of indigenous dadih lactic bacteria, especially some important colonization factors in GI tract, such as lysozyme, acid and bile tolerance. Bile Salt Hydrolase (BSH) activity, spectrum of bacteriocin, and antimutagenic activity of bacterial cells were also assessed. Twenty dadih lactic isolates were screened further for their tolerance to low pH, at pH 2 and 3 as well as their bile tolerance. There were ten isolates classified as acid and bile acid tolerant, and further screened for lysozyme tolerance, BSH activity. The spectrum of bacteriocin activity of isolates was assayed using cell-free neutralized supernatants by agar spot test against variety of pathogens. Lc. lactis subsp. lactis IS-10285, IS-7386, IS-16183, IS-11857 and IS-29862, L. brevis IS-27560, IS-26958 and IS-23427, Leu.mesen.mesenteroides IS-27526, and L. casei IS-7257 each has good survival rate at low pH values and in the presence of lysozyme, and short lag time in the presence of 0.3 % oxgall. Lc. lactis subsp. lactis IS-11857 and IS-29862 each has high BHS activity, Lc. lactis subsp. lactis IS-10285 and IS-16183 each had a positive spectrum of bacteriocin activity against E. coli 3301 and Lysteria monocytogenes ATCC 19112, while L. brevis IS-26958 has high BHS activity as well as positive spectrum of bacteriocin against E. coli 3301, Lysteria monocytogenes ATCC 19112, and S. aureus IFO 3060. All of the ten dadih lactic strains performed in vitro acid and bile tolerance, indicating a possibility to reach the intestine alive, and display probiotic activities. (Asian-Aust. J. Anim. Sci. 2003. Vol 16, No. 5 : 726-731)
Highlights
Metchnikoff (1907) observed that the consumption of fermented milks had beneficial effect associated with the auto-digestion of lactose
The result revealed that 10 strains of dadih lactic bacteria, Lc. lactis subsp. lactis IS10285, IS-7386, IS-16183, IS-11857 and IS-29862, L
0.3% oxgall (w/v) (Toit et al, 1998), and the other six strains of dadih lactic bacteria were considered as bile-salt tolerant with a lag time value in a range of 5.03-11.82 min (Table 2)
Summary
Metchnikoff (1907) observed that the consumption of fermented milks had beneficial effect associated with the auto-digestion of lactose. Lactic acid bacteria and their food products are thought to confer a variety of important nutritional and therapeutic benefits and have many documented health promoting or probiotic effects in human (Salminen et al, 1996) such as inhibition of pathogenic microflora, antimutagenic, and the reduction of cholesterol levels. Those lactic acid bacteria with scientifically supported health claims define as probiotic and have an increasingly high market potential. Many criteria have to be met, such as resistance to the enzymes in the oral cavity (e.g., lysozyme) and should have the ability to resist the digestion process in the stomach and the intestinal
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