Abstract
2′-fucosyllactose (2′FL) is one of the most abundant oligosaccharides in human milk, with benefits on neonatal health. Previous results point to the inability of the fecal microbiota from some infants to ferment 2′FL. We evaluated a probiotic formulation, including the strains Lactobacillus helveticus Rosell®-52 (R0052), Bifidobacterium longum subsp. infantis Rosell®-33 (R0033), and Bifidobacterium bifidum Rosell®-71 (R0071), individually or in an 80:10:10 combination on the microbiota and 2′FL degradation. Independent batch fermentations were performed with feces from six full-term infant donors of two months of age (three breastfed and three formula-fed) with added probiotic formulation or the constituent strains in the presence of 2′FL. Microbiota composition was analyzed by 16S rRNA gene sequencing. Gas accumulation, pH decrease and 2′FL consumption, and levels of different metabolites were determined by chromatography. B. bifidum R0071 was the sole microorganism promoting a partial increase of 2′FL degradation during fermentation in fecal cultures of 2′FL slow-degrading donors. However, major changes in microbiota composition and metabolic activity occurred with L. helveticus R0052 or the probiotic formulation in cultures of slow degraders. Further studies are needed to decipher the role of the host intestinal microbiota in the efficacy of these strains.
Highlights
20 Fucosyllactose Consumption in Fecal Cultures from Infants atNogacka 1,2, * , Silvia Arboleya 1,2 , Naghmeh Nikpoor 3 , Jeremie Auger 3 , Nuria Salazar 1,2 , Isabel Cuesta 1 , Jorge R
We have recently shown that the feeding mode and the intrinsic ability of the microbiota of two-month-old infants to degrade 20 -fucosyllactose (20 FL) are determinant factors influencing the evolution of microbiota composition and metabolic activity in fecal cultures [7]
A probiotic formulation and each of the constituting strains Lactobacillus helveticus Rosell® -52 (R0052), Bifidobacterium longum subsp. infantis Rosell® -33 (R0033), and Bifidobacterium bifidum Rosell® -71 (R0071) showed in fecal cultures of infants at two months of age, incubated in the presence of 20 FL, a differential effect on the intestinal microbiota composition and metabolic activity that was dependent on the specific probiotic strain and the intrinsic capacity of the microbiota of fecal donors to degrade 20 FL
Summary
Nogacka 1,2, * , Silvia Arboleya 1,2 , Naghmeh Nikpoor 3 , Jeremie Auger 3 , Nuria Salazar 1,2 , Isabel Cuesta 1 , Jorge R. Alvarez-Buylla 1 , Laura Mantecón 2,4 , Gonzalo Solís 2,4 , Miguel Gueimonde 1,2 , Thomas A. Tompkins 3 and Clara G. de los Reyes-Gavilán 1,2, *
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