Abstract

Abstract Potato plants free of viruses, bacteria and viroid could be maintained in vitro for a long time. Proper preparation of potato plants for in vitro culture provide its long-term storage in good condition. First step is to establish in vitro culture from young greenhouse grown plants in early developmental stage. Explants are maintained and propagated by nodal subculture on hormone-free Murashige and Skoog (MS) medium at temperature 20-22°C. After rooting, for longer preservation plants are maintained at temperature 8-10°C.

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