Abstract
This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.
Highlights
Introduction(Malpighiaceae) is native and endemic of Brazil (Seixas, Silva, Morais, & Santos, 2011)
The inoculations were performed in laminar flow chamber for maintaining the aseptic conditions, and the recipients were closed with polyvinyl chloride (PVC) film
With the results obtained in this experiment, we verified that the use of auxin IAA until the concentration of 1.0 μM showed effect negative in the in vitro multiplication of B. gardneriana
Summary
(Malpighiaceae) is native and endemic of Brazil (Seixas, Silva, Morais, & Santos, 2011). It is known in some regions of Northeastern Brazil, mainly in the Caatinga biome, as murici and murici pitanga. The fresh fruits are highly appreciated, being consumed in natura, as well as juices, popsicles, liquors, jellies, sweets, preserves and in the form of flour. Local populations live off extractive activities and, this species is one of their sources of income when commercialized in free markets (Donadio, Môro, & Servidone, 2002). In addition to the fruit, the remaining parts of the plant are widely used for therapeutic means, having some of the properties proven in laboratory (Sannomiya et al, 2005)
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