In vitro plantlet regeneration from cotyledon explants of Capsicum annuum L

Abstract

An efficient In vitro plant regeneration was established in Capsicum annuum L. from cotyledon explants. The best performance i.e., 80% of the explants responded for multiple shoot bud proliferation on MS medium containing 10 mg/l Zeatin. The maximum number of buds per explants was 50.28 ± 2.69 and the bud length of 0.5 ± 0.22 cm was obtained in this medium after 30 days. The average number of 80.32 ± 0.16 multiple shoot bud and the average shoot bud length of 1.0 ± 0.19 cm was attained on the same medium after 90 days. These shoot buds on transfer to MS + 10 mg/l Zeatin + 2.0 mg/l GA3 yielded 6.3 ± 0.35 number of multiple shoots with an average shoot length of 4.6 ± 0.62 cm after 30 days. Shoots rooted well (80% of induced shoots) on half strength MS medium supplemented with 0.5 mg/l IBA. The average number of roots per shoot was 8.2 ± 0.79 and the average root length of 4.5 ± 0.58 cm was recorded. About 80% of In vitro derived plantlets were survived under open field conditions.