In Vitro Plant Regeneration through Different Explant of Trichosanthes cucumerina L.

Abstract

An efficient in vitro plant regeneration protocol was developed for Trichosanthes cucumerina L. from leaf and shoot tip explant. Explants were cultured on MS medium supplemented with 6-benzyl amino-purine (BAP) thidiazuron (TDZ) and kinetin (Kin) ranging from 0.5-3.0 mg/L alone and in combination with gibberellic acid (GA3) ranging from 0.2-1.0 mg/L and produced multiple shoots. The highest percentage of response 96.3 percentage of response in leaf and 39.33 percentage of response in shoot tip explant were noticed on 0.6 mg/L GA3 in combination with 2.5 mg/L BA. The 84.38 and 24.96 percentage of response were noticed in leaf and shoot tip explants respectively in 2.5 mg/L. The regenerated shoots were then transferred to MS medium fortified with different concentrations of indole acetic acid (IAA), 1-naphthalene acetic acid (NAA) and indole butyric acid (IBA) (0.5-3.0mg/L) alone for root induction. The maximum percentage of response 96.63 with 9.66 cm root length per shoot was obtained from 1.5 mg/L NAA. The rooted plantlets were successfully acclimatized in red soil, farmyard manure and sand mixture and subsequently established in the field with 90% survival.