In vitro plant regeneration of potato (Solanum tuberosum L.) at the rate of different hormonal concentration

Koushik Khan,Md Abdur Rauf Sarkar,Md Faruk Miya,Sayeed Shahriyar,Soleh Akram

doi:10.3329/ajmbr.v1i2.25625

Abstract

One of the goals of the experiment is to standardization of HgCl2 treatment for explants sterilization. The objectives also include developing a reproducible cost effective protocol for large scale production of Solanum tuberosum of Cardinal variety plantlets from selectively better clones through plant in vitro propagation methods. Selection of growth regulators for proper multiple shoots regeneration, elongation and root induction. To produce genetically uniform plantlets within a short time capable surviving in natural condition raised in in vitro environment. Shoot tip and nodal segment explants from field grown plants were used as experimental materials in this investigation. All explants were cultured on Murashige and Skoog medium supplemented with various plant growth regulators. For surface sterilization of explants, HgCl2 (0.1%) for 2 minutes was found to be most effective for complete destroying of surface pathogens and getting healthy tissues. Shoot regeneration was observed from both shoot tips and nodal explants for the studied plant. Maximum number of shoot per culture (17) was recorded and it also obtained the highest average length of the shoot (5cm) in Murashige and Skoog medium containing no hormone. On the other hand 6-benzyl amino purine (0.2mg/l) in 3 media showed the highest rate of shoot multiplication (73%) and the highest average length (4cm). In case of Gibberellic acid (0.1mg/l) in Murashige and Skoog media showed its highest rate of shoot regeneration (82%) and the highest average length (4.5cm). From the overall experiment it was observed that shoot tips are more responsive for micro propagation. In root induction Murashige and Skoog medium supplemented with different concentration (0.5, 1, 1.5 and 2mg/l) of indol-3-acetic acid and kinetin. Indol-3-acetic acid and kinetin (1.5+1.5 mg/l) showed its lowest rate of root regeneration (40%) and the average length of the root (1.5 cm). On the contrary Murashige and Skoog medium with no hormone showed the rate of root regeneration (96%) and the highest average length of the root (2.5 cm). The supplemented Murashige and Skoog media with no hormone showed the best performance for root regeneration.Asian J. Med. Biol. Res. June 2015, 1(2): 297-303

Highlights

MethodsIn the present experiment different culture media with various growth regulators and additive were used for shoot tip and nodal segment culture: (a) Murashige and Skoog (Murashige and Skoog, 1962) medium with different concentrations of 6-benzyl amino purine, Gibberellic acid singly was used for shoot induction .For carbon source 3% sugar was used and the medium was solidified with 6-6.2% agar.(b) Half and full strength of medium with different concentration of indol-3-acetic acid and kinetin were used for root induction
Potato (Solanum tuberosum L.) is a tuber-bearing tetraploid species belonging to the Solanaceae family
Shoot tips and nodal segments were used for micro propagation of Solanum tuberosum a cultivar of cardinal

Summary

Methods

In the present experiment different culture media with various growth regulators and additive were used for shoot tip and nodal segment culture: (a) Murashige and Skoog (Murashige and Skoog, 1962) medium with different concentrations of 6-benzyl amino purine, Gibberellic acid singly was used for shoot induction .For carbon source 3% sugar was used and the medium was solidified with 6-6.2% agar.(b) Half and full strength of medium with different concentration of indol-3-acetic acid and kinetin were used for root induction. The photoperiod was maintained generally 16 hours and 8 hours dark The culture vessels such as test tube, bottle conical flask, measuring cylinders, glass rods, beakers, pipette pumps, parafilm, cotton plug, rubber bands, filter paper, aluminum foils, forceps, fire box, marker pen, spirit lam, needle, sharp blade, stereomicroscope, electronic balance, autoclave, pH meter, magnet stirrer, laminar airflow machine etc. Data were recorded on the following parameters, different time duration for surface sterilization of explants, days to shoot regeneration, number of shoot per explants, days to root induction, number of root per explants

Results

Discussion

Conclusion