Abstract
Plant regeneration from mature zygotic embryo culture of Iris reichenbachii was studied. The effect of 2,4-dichlorophenoxyacetic acid (2,4-D, 0-10 mg L -1 ) on induction of morphogenesis in vitro is investigated. The best response for callus induction is observed on MS medium with 10 mg L -1 2,4-D. White embryogenic, yellow non-embryogenic and green organogenic calli are observed. The fresh weight, as well as, percentage portion of embryogenic and organogenic calli is 2,4-D dependent. Shoots are succesfuly multiplied by adventitive organogenesis from organogenic callus. Germination of somatic embryos and rooting of shoots were achieved on MS solid medium without hormones.
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