In vitro Phosphate Solubilization by Bacillus sp. NPSBS 3.2.2 Obtained from the Cotton Plant Rhizosphere

Abstract

Cotton is one of the important cash crops of India. Apart from being an important source of fibres, cotton seeds also form an important source of edible oil. A lot of Phosphatic fertilizers are used to during cotton cultivation but a major portion of this Phosphate gets converted into insoluble form which is not available to the plants. However, there are certain Phosphate Solubilizing Bacteria which can solubilize inorganic insoluble phosphorous to inorganic soluble form and this form of phosphate can be easily used by plants. In present study isolation of Phosphate Solubilizing Bacteria was carried out from rhizosphere soil sample of cotton plants growing in various villages of District Mahendergarh, Haryana. A total of 15 bacterial isolates were obtained which were subjected to primary and secondary screening. On the basis of secondary screening NPSBS3.2.2 was found to solubilize the maximum amount of Phosphorous (0.129¼g/ml). The isolate was grown in different conditions and it was found that sucrose was the most suitable Carbon source at pH 7 at the incubation time period of 96 hours. The isolate was characterized morphologically, biochemically and on the basis of 16s rDNA sequencing (Genebank Accession Number- KF974682) and was found to be a member of the genus Bacillus.