In vitro Permeation Study of Ketoprofen Patch with Combination of Ethylcellulose and Polyvynil Pyrrolidone as Matrix Polymers

Abstract

Objective: Transdermal patch can release drug to provide systemic pharmacological effect. Matrix used in the patch formula influences the release of the drug. The aim of the study was to evaluate permeation of Ketoprofen patch containing combination of Ethyl Cellulose (EC) and Polyvynil Pyrrolidone (PVP) as matrix. EC is a hydrophobic polymer while PVP is a hydrophilic one. Transdermal formulation containing two polymers with different polarity will provide better permeation results than use only one type of polymer. Ketoprofen 1% was used as a model of active substance in the study. Methods: Ratio of EC and PVP concentration (1:1, 1:3 and 3:1) in patch formula was determined using Simplex Lattice Design (SLD) method. In vitro permeation study was done for 12 h using Franz diffusion cell and shed snake of Phyton reticulatus as a membrane. Amount of the drug released was analyzed using Spectrophotometer UV-Vis (λ = 262 nm). The drug release mechanism was known by determination the drug release kinetics order. Results: The study result showed that the higher PVP concentration was used in the formula, the higher percentage of Ketoprofen permeation was obtained. Patch formula containing EC:PVP in ratio 1:3 had the highest percentage of permeation (93.66%). The drug release kinetics of Ketoprofen from the patch followed zero order kinetics. Conclusion: Combination of EC and PVP in ratio 1:3 resulted the highest percentage of ketoprofen permeation for 12 h (93.66%). The drug release mechanism followed zero order kinetics. Key words: Ethylcellulose, Ketoprofen, Patch, Permeation, Polyvynil pyrrolidone, Simplex Lattice Design.