Abstract
The aim of this study was to evaluate the viability of periodontal ligament cells of avulsed teeth in three different storage media. Forty-five mature premolars extracted for orthodontic therapeutic purposes were randomly and equally divided into three groups according to the storage medium: milk (control), rice water and egg white. After placing extracted teeth for 30 min in storage media, the scrapings of the periodontal ligament (PDL) were collected in Falcon tubes containing collagenase in 2.5 mL of phosphate buffer saline and were incubated for 30 min and centrifuged for 5 min at 800 rpm. Cell viability was analyzed by Trypan blue exclusion. Rice water had a significantly higher number of viable cells compared to egg white and milk. There was no statistically significant difference between egg white and milk. Rice water may be able to maintain PDL cell viability of avulsed teeth better than egg white or milk.
Highlights
Avulsion or exarticulation is the complete displacement of a tooth from its alveolar socket due to traumatic injury [1]
Milk, Hank’s balanced salt solution (HBSS), α Minimum essential medium (MEM), propolis, Viaspan, soy milk and many more storage media have been proposed for storing avulsed teeth [5,6,7]
HBSS is recommended by the American Associations of Endodontists (AAE) as a storage medium of choice for treatment of avulsed teeth because of its ability to provide long-term preservation of periodontal ligament (PDL) cell viability
Summary
Avulsion or exarticulation is the complete displacement of a tooth from its alveolar socket due to traumatic injury [1] It is one of the most complex forms of dental injury, which may occur as a result of fight and sports in the permanent dentition, while falls against hard objects is a frequent cause in the primary dentition [2]. Exarticulation leads to injury to periodontal ligament tissues along with severance of the neurovascular bundle of the dental pulp at the apical foramen resulting in pulp necrosis. In such condition, the tooth should be maintained in a suitable medium until it is replanted by a dentist as soon as possible [3]. There is need to identify a medium that is readily available and yet effective as well
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