In vitro performance of lipid-PLGA hybrid nanoparticles as an antigen delivery system: lipid composition matters.

Yun Hu,Kristel Fuhrman,Marion Ehrich,Chenming Zhang

doi:10.1186/1556-276x-9-434

Yun Hu, Kristel Fuhrman + Show 2 more

Open Access

https://doi.org/10.1186/1556-276x-9-434

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Journal: Nanoscale Research Letters	Publication Date: Aug 27, 2014
Citations: 71	License type: cc-by

Affiliation: Virginia Tech

Abstract

Due to the many beneficial properties combined from both poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) and liposomes, lipid-PLGA hybrid NPs have been intensively studied as cancer drug delivery systems, bio-imaging agent carriers, as well as antigen delivery vehicles. However, the impact of lipid composition on the performance of lipid-PLGA hybrid NPs as a delivery system has not been well investigated. In this study, the influence of lipid composition on the stability of the hybrid NPs and in vitro antigen release from NPs under different conditions was examined. The uptake of hybrid NPs with various surface charges by dendritic cells (DCs) was carefully studied. The results showed that PLGA NPs enveloped by a lipid shell with more positive surface charges could improve the stability of the hybrid NPs, enable better controlled release of antigens encapsulated in PLGA NPs, as well as enhance uptake of NPs by DC.

Highlights

Nanoparticles made from poly(lactic-co-glycolic acid) (PLGA) or lipids have been used as drug delivery systems for many years
In PK NP preparation, 3 mg of keyhole limpet hemocyanin (KLH) was added into 200 mg PLGA during the primary emulsion, and the results indicated that around 75% of the KLH was entrapped inside PLGA
Such an increase in size is probably caused by the addition of a lipid layer on the Components of NPs KLH DOTAP DOPC DSPE-polyethylene glycol (PEG)

Summary

Introduction

Nanoparticles made from poly(lactic-co-glycolic acid) (PLGA) or lipids have been used as drug delivery systems for many years. PLGA and liposome nanoparticles (NPs) share some common merits, such as long circulation time, biocompatibility, tunable size, and high drug loading capacity [1,2]. The surface chemistry of liposomes can be tuned to meet different requirements by adjusting the types or in the past decade, lipid-PLGA hybrid NPs have exhibited great potentials as a delivery system for cancer drugs, antigens, as well as in vivo imaging agents. They may play an important role in overcoming the increasingly prevalent multidrug resistance (MDR) [8]

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