In Vitro Penetration into Bovine Oocytes and Zona-Free Hamster Oocytes by Bull Spermatozoa Treated with Liposomes

Abstract

The objectives of this study were 1) to determine whether in vitro penetration of bovine and zona-free hamster oocytes, using spermatozoa treated with the lipid dilauroylphosphatidylcholine, was correlated with the fertility of bulls and 2) to determine whether utilizing results from several laboratory assays could effectively evaluate fertility. Cryopreserved semen was used from 12 bulls having lifetime nonreturn rates ranging from 66 to 81%. The lipid concentration that maximized penetration of hamster oocytes was determined as well as the number of spermatozoa entering all hamster oocytes, number of spermatozoa in each penetrated hamster oocyte, fertilization of bovine oocytes, blastocyst development of bovine oocytes, and the percentage of motile spermatozoa of each sample. Correlations were low between the lipid concentration that maximized each individual parameter and nonreturn rate (r≤0.34). The six-parameter regression equation accounted for 71% of the variation in bull fertility, and a four-parameter equation accounted for 61% of the variation in fertility. Pooling data for pairs of bulls into six fertility groups to increase information per data point still resulted in poor predictions of fertility for individual parameters. However, a four-variable model, including the penetration rates for hamster and bovine oocytes, the number of spermatozoa that penetrated hamster oocytes, and the percentage of motile spermatozoa, accounted for 98% of the variability in bull fertility.