Abstract
Regeneration was induced in the matured skeletal muscles of adult mice by intramuscular injection of hypertonic saline. After passing through the degeneration phase, the regeneration of muscle took place and proceeded with considerable synchrony. Regenerating myogenic cells were isolated from the saline-treated muscles by cold trypsinization with the aid of Yaffe's selective plating procedure [Yaffe, D. (1968) Proc. Nat. Acad. Sci. USA 61, 477–483]. The myogenic cells thus isolated readily developed into muscle fibers possessing cross striations and the ability to contract when cultured in vitro.
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