In vitro multiplication of wild Manihot species with different naphthaleneacetic acid and benzylaminopurine concentrations

Karen Cristina Fialho Dos Santos,Jucieny Ferreira De Sá,Deyse Maria De Souza Silveira,Antônio Da Silva Souza,Carlos Alberto Da Silva Ledo,Mariane De Jesus Da Silva De Carvalho Carvalho

doi:10.4025/actascibiolsci.v43i1.52866

Abstract

In vitro multiplication is an important tissue culture technique that is capable of efficiently producing seedlings at any scale. It is a propagation method based on the aseptic culture of small propagules in a suitable culture medium to enable plant regeneration. Multiplication experiments conducted in vitro to set protocols adapted to wild Manihot species have used modified mineral salts and MS vitamins as basic culture medium. Here, 25 treatments based on combinations of the regulators benzylaminopurine (BAP) and naphthaleneacetic acid (NAA) at 0, 0.025, 0.05, 0.075, and 0.1 mg L-1 were used for in vitro multiplication of three genotypes of wild Manihot species (M. violaceae Pohl Müll. Arg., M. pseudoglaziovii Pax & Hoff., and M. flabellifolia Pohl). Plant height and the number of 1 cm minicuttings, number of roots, shoots, green leaves and senescent leaves were recorded 120 days after explant inoculation. M. violaceae Pohl. Müll. Arg. and M. flabellifolia Pohl. presented favorable results with 0.05 and 0.025 mg L-1 NAA, respectively. Culture medium lacking NAA and BAP favored the in vitro growth of M. pseudoglaziovii Pax & Hoff.

Highlights

In vitro micropropagation is one of the tissue culture techniques that can be an alternative to conventional vegetative propagation
In vitro micropropagation has many advantages compared to conventional vegetative propagation methods
This study investigated the use of MS medium supplemented with different concentrations of naphthaleneacetic acid (NAA) and BAP to establish in vitro multiplication protocols suitable for three wild Manihot species

Summary

Introduction

In vitro micropropagation is one of the tissue culture techniques that can be an alternative to conventional vegetative propagation. These two growth regulators are widely used in combination, and their interaction often favors plant multiplication and development in vitro. This study investigated the use of MS medium supplemented with different concentrations of NAA and BAP to establish in vitro multiplication protocols suitable for three wild Manihot species.

Results

Conclusion