Abstract
Complete plantlets were regenerated in vitro from root explants of Strychnos potatorum Linn.f. (Chilla) on Murashige and Skoog’s (MS) medium supplemented with various concentrations of cytokinins. Multiple shoot induction occurred in root explants on MS medium supplemented with 1-4 mgl-1 benzylaminopurine or Kinetin. Maximum numbers of shoots/explant (18.4 + 0.2) were induced on MS medium fortified with 2 mgl-1 BAP. Shoots obtained from BAP and Kn amended media developed roots in 100% cultures on half strength MS basal medium fortified with Indole butyric acid (1 mgl-1). Hardening and establishment of regenerated plantlets was achieved in green house.
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