In vitro multiplication of rough lemon (Citrus jambhiri Lush.)

Abstract

In vitro multiplication of Rough Lemon (Citrus jambhiri Lush.) was carried out from nodal segments to standardize the protocol for mass multiplication under lab conditions. It was found during experimentation that MS medium supplemented with BAP (1.5 mg/L) and malt extract 500 mg/L resulted in maximum culture establishment, number of shoots and length of longest shoot per culture in minimum time during shoot proliferation. The in vitro multiplied shoots could be best rooted in half strength medium supplemented with IBA and NAA (1.0 mg/L) each with 3 % sucrose as carbohydrate source. In vitro formed plantlets were hardened in potting mixture containing sand, soil and FYM (1:1:1) and highest survival (83.33 %) was achieved after transplantation when rooted plantlet leaves were treated with 50 % glycerol as an antitranspirant.