Abstract
A protocol for micro propagation for known sexuality of Jojoba was developed and standardized. Nodal explants from actively growing young shoots were used to proliferate in MS medium supplemented with different concentrations of cytokinins and auxins. Pre-treatment with bavistin (0.2% for 3 min.) followed by HgCl2 (0.1% for 5 min.) was found to be the best for disinfecting the explants. Maximum proliferation in nodal explants was recorded on MS medium containing 2.0 mg l-1 BAP. The medium having MS + 0.5 mg 1-1 BAP + 0.1 mg 1-1 IAA produced maximum number of shoots (5.7) per explant. Elongation of in vitro multiplied shoots was found maximum in MS + 2.0 mg 1-1 BAP. The highest rooting (63.7 percent) was observed in full strength MS medium supplemented with 0.5 mg 1-1 IBA. The in vitro raised plants were hardened for survival after pricking out in different potting media kept in a mist chamber with gradually decreasing humidity regime. Sand, soil and vermicompost mixture in ratio of 3:1:1 was found suitable and recorded 83 percent survival of in vitro raised clonal plants.
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