In Vitro Multiplication, Conservation and Ex Vitro Acclimation of Drosera rotundifolia

Abstract

At the Fruit Research Station of Cluj experiments were carried out for the optimization of in vitro proliferation of Drosera rotundifolia as well as for the in vitro conservation, in vitro rooting and ex-vitro acclimation of this species. For proliferation, modified Murashige & Skoog (MS) media containing either 200 ml/l coconut water or 5 mg/l Kinetin proved to be optimal. BAP in the media proved not to be suitable. For in vitro conservation a double layer hormone-free MS medium was used, which ensured the long-term storage and in vitro proliferation of Drosera rosettes. For in vitro rooting, two variants of MS medium were used, one with macroelements diluted to ¼ and the other with macroelements diluted to ½. Both variants proved to be suitable for in vitro rooting and increasing plantlet size; they provided 100 % rooting percentage and there were no significant differences between the two variants regarding the biomass of the resulting plantlets. Ex-vitro acclimation was done most efficiently by hydroculture, in uncovered trays containing water with neutral pH.