In vitro multiple shoot proliferation and plant regeneration in rose(Rosa hybrida L.)

Abstract

This study was conducted to investigate an optimal condition for shoot proliferation and regenerate shoots from in vitro leaflet and embryogenic calli from in vitro roots in rose. The effect of BAP on shoot proliferation was somewhat different depending upon genotypes or gelling agents. Leaflets with petiole cut from donor shoots which had been cultured in MS medium supplemented with 0.1 <TEX>$mg{\cdot}L^{-1}$</TEX> NAA for six weeks was effective for regeneration of adventitious buds(ABs) as well as shoot elongation of Rosa hybrida cv. Sweet Pink. Culturing seven leaflet explants per petri plate(<TEX>$100mm{\times}15mm$</TEX>) was effective for regeneration of ABs. Embryogenesis was shown in the calli induced from roots of Rosa hybrida cv. Sweet Pink cultured in the SH medium supplemented with 11 <TEX>$mg{\cdot}L^{-1}$</TEX> 2, 4-D for four weeks. Color of calli induced from roots was yellow although their color was a little different as type of basal medium.