In Vitro Multiple Shoot Induction From Embryonic Axes of Annual Herbaceous Legume Faba Bean (Vicia faba L.)

Ferzat Turan

doi:10.47115/bsagriculture.1063435

Abstract

The faba bean (Vicia faba L.) is cultivated in the Mediterranean and Aegean regions only. It is a rich source of protein and an important source of food and feed for human and animal consumption. Faba beans have a narrow genetic base and their improvement through hybridization is not feasible because of high pollen self-incompatibility. This study was carried out using the embryonic axis of cv. Filiz99 and Eresen87 regenerated on MS medium containing 11 different combinations of BAP and NAA. The results showed 100% shoot regeneration frequency with maximum number of 3.3 and 3.5 shoots per explant on cv. Filiz99 and Eresen87 respectively. Regenerating shoots were rooted on 1 mg L–1 IAA. The in vitro regenerated shoots were continuously cultured for 3 weeks to acclimatize them. This approach could improve broad bean seed germination and subsequently regeneration. The results could also facilitate genetic transformation studies.

Highlights

Faba bean (V. faba L.) is a herbaceous multipurpose herbaceous legume plant grown in many parts of the world (Duke 1981)
A few reports on tissue culture of faba bean are available, there has been no report of tissue culture from Turkey and it is an important issue
The results showed that SOD, CAT, and POD enzymatic activities of non-water-soaked seedlings of both cultivars were higher compared to these activities on seedlings obtained after water soaking

Summary

Introduction

Faba bean (V. faba L.) is a herbaceous multipurpose herbaceous legume plant grown in many parts of the world (Duke 1981). Like other faba beans (Rowlands 1964), these are less genetically diversified cultivars and face problems of partial self-incompatibility that more often results in the collapse of their fertile ovules This has led to slow progress in broad bean breeding and varietal improvement activities in Turkey (Bond, 1987; Selva et al, 1989; Bahgat et al, 2009). More often the problem of partial self-incompatibility results in the collapse of fertile ovules in broad bean resulting in genetically less diversified plants (Rowlands, 1964; Stoddard and Bond, 1987) This has led to slow progress in broad bean breeding and varietal improvement activities (Bond, 1987; Selva et al, 1989; Bahgat et al, 2009). Establishing faster regeneration systems can be used in BSJ Agri / Ferzat TURAN

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Discussion

Conclusion