Abstract
The morphogenetic response of Ligusticum porteri was investigated as a part of a conservation strategy and was compared to that of Petroselinum crispum. After in vitro germination of seeds, plantlets were excised into different explants and cultured in an induction medium supplemented with 2,4-dichlorophenoxyacetic acid (0–18.09µM) or α-naphthaleneacetic (0–21.48µM) acid in combination with 6-benzylaminopurine (0–13.31µM) to induce callus formation. GC-MS analysis of calli, mature aerial parts and roots extracts of both species led to the identification of 3-butylidenephthalide (1), 3-n-butylphthalide (2) and 3,6,7,-trimethoxy-isobenzofuran-13(H)-one (3). This is the first report on phthalides production from in vitro cultures of L. porteri.
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