In vitro migration of peritoneal and spleen cells and its inhibition in some avion species

Abstract

Cell migration and its inhibition was tested by the capillary tube technique with peritoneal exudate cells and spleen cells of chicken, turkey, goose, guinea fowl, and Japanese quail. Peritoneal cells were produced by ip administration of proteose peptone and harvested 24 hr later. Liquid paraffin proved to be unsatisfactory for preparation of peritoneal cells in some avian species. Mononuclear cells represented no more than 50–60% of the peritoneal cell populations, the other 50% being polymorphonuclear cells in all five avian species studied. Cell migration was demonstrated with chicken, turkey, and goose peritoneal and spleen cells, but not with those of guinea fowl and Japanese quail. The composition of the cell populations in the migration areas was nearly the same as in the initial preparations of peritoneal and spleen cells. Spleen cell migration was inhibited to a greater extent than that of peritoneal cells. Migration inhibitory factor (MIF) produced by chicken and turkey lymphocytes exhibited some species specificity.