In vitro Micropropagation of Duckweed (Lemna minor L) Plant with Temporary Immersion System Bioreactors

Abstract

Which is very rich in protein in plant Lemna minor L. abundant in Turkey ecologically plays a very important role in protecting the elimination of water pollution and aquaculture environment balance. In this study, in vitro propagation of this plant with Temporary Immersion System Bioreactor and determination of the effects of used plant growth regulators on the protein content of the plant were aimed. With this objective different variant of media with and without sucrose, varying pH and concentrations of BAP, kinetin, TDZ in medium were analyzed. Experiments for micropropagation were performed for 8 hours in the dark and 16 hours in white fluorescent light (150 µ mol photons m-2s-1) under photoperiod and at 24 ± 1 ° C. The highest plant growth was observed at pH 7.23 in sugar free liquid MS medium containing 0.2 mg/L BAP. 50.44 plants per explant were recorded in this medium. In addition, the maximum number of plants per explant in liquid MS medium containing 0.05 mg/L kinetin was calculated as 57,823 and the maximum number of plants per explant in liquid MS medium containing 0.6 mg/L TDZ was calculated as 50.74. As a result of nitrogen determination studies with Kjeldahl method, the protein value of the plant was determined as 25.5%. As a result of hormone application, it was seen that protein content in plant increased to 29.18% with 0.5 mg/L BAP. It was concluded that the aim of the study were fulfilled and positive effects of Temporary Immersion System Bioreactors on plant multiplication were found.