In vitro micropropagation of apple (Malus domestica) through Axillary buds and shoot apices culture

Abstract

The objective of the study was in vitro micro-propagation through axillary buds and shoots peaks culture of each root stock with randomized design in the three replications. For callus inductions lateral apples have been cultured on MS medium contained 30 g/L sucrose, 7 g-L agar, vitamins and supplemented with 1, 2, 3 and 4 mg-L of NAA mixed with 2.0 mg-L of Kn were examined. In shoot multiplication, MS media fortified with 0.5, 1.0, 1.5 and 0.2 of mg-L of BAP and 0.5, 1.0, 1.5 and 0.2 of mg-L of Kn and mixtures of 0.5 mg-L BAP with 0.5 mg-L of Kn and 2 mg-L BAP with 0.5 mg-L of Kn have been examined. The data shows that treatments with 0.5mg-L has shoots growths were decreased compared to other treatments. In root induction, two groups of media MS 0.25, 0.5, 0.75 1.0 mg-L of AIB, and 0.25, 0.5, 0.75, 1.0 mg-L of NAA were used for evaluations and higher roots length were recorded at 0.25 mg-L of IBA. In conclusion, it is recommended to use in vitro propagation protocols for fruit multiplication in nursery to farmers to produce a large amount of seedling within a short period of time