In Vitro Micropropagation of Aloe adigratana Reynolds Using Offshoot Cuttings.

Mulu Niguse,Haftom Baraki Abraha,Desta Berhe Sbhatu

doi:10.1155/2020/9645316

Mulu Niguse, Haftom Baraki Abraha + Show 1 more

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https://doi.org/10.1155/2020/9645316

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Journal: TheScientificWorldJournal	Publication Date: Apr 16, 2020
Citations: 9	License type: CC BY 4.0

Affiliation: Mekelle University

Abstract

This study aimed at developing a suitable and reproducible protocol for in vitro micropropagation of Aloe adigratana Reynolds using explants from offshoots with the help of the most commonly used plant growth regulators (PGRs). Explants were initiated in full-strength Murashige and Skoog (MS) media enriched with 0.2 mg/L benzylaminopurine (BAP) + 0.2 mg/L naphthaleneacetic acid (NAA). Shooting experiment was conducted in full-strength MS media enriched with 0/0, 0.5/0.5, 1.0/0.5, 1.5/0.5, and 2.0/0.5 mg/L BAP/NAA. Likewise, rooting experiment was carried out in half-strength MS media enriched with NAA at 0.5, 1.0, and 1.5 mg/L and indol-3-butyric acid (IBA) at 0.5, 1.0, and 1.5 mg/L. Finally, acclimatization study was conducted in greenhouse, nursery, and open field. The shooting response of the plant was best in MS media enriched with 1.0 mg/L BAP + 0.5 mg/L NAA. This media formulation resulted in the shortest mean number of days to shooting (14.00 ± 2.30 days), the highest mean shoot number (4.00 ± 3.40), and the second highest mean shoot length (8.60 ± 2.10 cm). IBA enhanced rooting at higher concentrations (1.0 and 1.5 mg/L), but NAA did the same at lower concentrations (0.5 and 1.0 mg/L). All plantlets (n = 62) survived primary acclimatization. Secondary acclimatization in composted and matured soil media under nursery and open field (sun light) condition produced 88 to 93% survival rate. The death of plantlets in the secondary acclimatization is accounted to mechanical damage. This study demonstrated that the tested PGRs were useful in enhancing the in vitro micropropagation of the plant. Future studies need to focus on optimizing the protocol for large-scale, commercial micropropagation.

Highlights

Aloe L. (Aloaceae) species are succulent flowering plants, highly valued in many communities of the world. e ethnobotanic, ethnomedicinal, and ethnoveterinary values of aloes are well-documented
Shoot initiation experiment was aimed at producing healthy and live explants. It was tested in full-strength Murashige and Skoog (MS) media enriched with 0.20 mg/ L BAP and 0.20 mg/L naphthaleneacetic acid (NAA). irty (30) explants were cultured for 10 days. is experiment resulted in 27 (90%) healthy and live explants
In vitro micropropagation studies with many other Aloe species reported that shoot-yielding structures or microshoots are initiated within few day of culturing [11, 15, 21,22,23,24,25,26,27]. ese researchers showed that application of various combinations and concentrations of BAP and auxins like IAA, indol-3-butyric acid (IBA), and NAA help in formulating the best initiation media

Summary

Introduction

Aloe L. (Aloaceae) species are succulent flowering plants, highly valued in many communities of the world. e ethnobotanic, ethnomedicinal, and ethnoveterinary values of aloes are well-documented. (Aloaceae) species are succulent flowering plants, highly valued in many communities of the world. E ethnobotanic, ethnomedicinal, and ethnoveterinary values of aloes are well-documented. Many Aloe species are extensively used in the preparation of cosmetic and toiletry industries. Aloes grow nearly in all parts of the world [1,2,3,4]. Different accounts put the number of Aloe species between 450 and 600. Ethiopia and Eritrea are believed to be home to 50 species of Aloe where 31 are endemic [3, 5,6,7]. Ethiopian and Eritrean aloes grow in various soil types and altitudes stretching from the sea level at Massawa (Aloe eumassawana Carter, Gilbert & Sebsebe) to ca. Many of them are restricted to very small geographic areas and ecological zones [3, 6]

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