In vitro micro-tuberization in Bunium persicum with different media and sucrose concentrations

Abstract

Bunium persicum (Boiss.) B. Fedtsch. is one of economically important member of the Apiaceae family with a tuberous root. Production of this plant is restricted due to seed dormancy and long juvenile time. The objective of this study was to determine the best media and optimum concentration of sucrose for in vitro microtuberization of Black Zira. Therefore, different basal media (Murashige and Skoog (MS), Woody Plant Medium (WPM), Gamborg’s B5, and Gupta and Durzan’s DCR) with 3% sucrose were used for microtuber induction. Then best media was tested by varying concentrations of sucrose (3, 6, 9, and 12%) to increase morphological characteristics diameter and weight of microtubers. The experiment was done in a Randomized Complete Block Design with four treatments and 8 replications per treatment. According to the results obtained in this study, the highest mean of the root length (4.5196 cm), tuber length (1.175 cm), tuber width (0.3575 cm), tuber diameter (4.1 mm), and tuber weight (0.1472 g) after six weeks was observed in the WPM medium. Among the four concentrations of sucrose in WPM medium supplemented with 6% sucrose indicated a better response than the other concentrations in terms of microtuber diameter (7.675 mm), and weight (0.2687 g) average and was found optimum. Therefore, the WPM was recognized as the best medium for micro-tuberization.