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Abstract
Stimulation of single rat anterior pituitary glands has been obtained in a continuous superfusion system as demonstrated by radioimmunoassay of LH in the superfusate. The results show the possibility of repeatedly stimulating a single pituitary gland, with approximately graded response to hypothalamic extract (H.E.), in the presence of a constant basal release rate. The data indicate that a relatively constant release rate is obtained under these conditions and that intermittent administration of H.E. results in repeated acute release of lutenising hormone (LH). The period of release was largely limited to the time during which H.E. was administered. Equivalent amounts of cerbral cortical extract only produced a slight increase of LH concentration in the superfusate.
Published Version
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