Abstract
The major actions of undigested carbohydrates, such as non-starch polysaccharides and resistant starch, on the physiology of man are related to colonic fermentation. It is very difficult to study fermentation in vivo.In vitromodels are often used but these models differ in almost every aspect even when only the simplest batch cultures of human faeces are considered. Factors such as composition of the medium, buffering capacity, inoculum concentration, and fermentation time vary considerably making direct comparison of the results from different studies impossible. The authors have devised a standardin vitrofermentation method for resistant starch and tested it in eight laboratories with a total of 40 individual faecal inocula. This simple method was well received and taking biological variation into account yielded comparable results for 24 h short chain fatty acid production and residual starch in all but one laboratory.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
