In vitro membrane stabilizing, thrombolytic and antioxidant potentials of Drynaria quercifolia L., a remedial plant of the Garo tribal people of Bangladesh.

Farjana Rahman Chaity,Mahbuba Khatun,Mohammad Sharifur Rahman

doi:10.1186/s12906-016-1170-5

Farjana Rahman Chaity, Mahbuba Khatun + Show 1 more

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https://doi.org/10.1186/s12906-016-1170-5

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Abstract

BackgroundDrynaria quercifolia L. (Family- Polypodiaceae) is a fern grows in Bangladesh used in traditional healing by the Garo tribe of Mymensingh district. In the current study, rhizomes and fertile foliage fronds of this plant have been investigated comprehensively to assess their in vitro membrane stabilizing, thrombolytic and antioxidant properties.MethodsRhizomes and fertile foliage fronds of D. quercifolia were collected, dried, powdered and extracted with methanol. Later on, crude methanol extracts of the plant parts were fractionated into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions. The extractives were then subjected to membrane stabilizing, thrombolytic and antioxidant assays.ResultsIn membrane stabilizing assay, crude methanol extracts of rhizomes and fertile foliage fronds and their petroleum ether fractions were found to be very effective for stabilizing erythrocyte membrane in hypotonic solution. In case of thrombolytic study, crude methanol extract of rhizomes and its aqueous fraction exhibited noticeable clot lysis. However, in antioxidant assays, crude methanol extracts of the tested plant parts and their aqueous fractions exhibited potent 1,1-diphenyl-2-picrylhydrazyl (DPPH), hydrogen peroxide and 2, 2’-azinobis (3-ethylbenzothiazoline sulphonic acid) (ABTS) radical scavenging activity. Besides, these extractives also displayed substantial ferric reducing potential in ferric reducing antioxidant power (FRAP) assay. Crude methanol extracts of the plant parts and their aqueous fractions were also found rich in phenolics.ConclusionThis study demonstrates the medicinal potentials of D. quercifolia and justifies the local uses of it by the Garo tribal people of Bangladesh for multiple disease management.

Highlights

Drynaria quercifolia L. (Family- Polypodiaceae) is a fern grows in Bangladesh used in traditional healing by the Garo tribe of Mymensingh district
The aim of the present study is to investigate the rhizomes and the fertile foliage fronds of D. quercifolia for their membrane stabilizing, thrombolytic and antioxidant activities comprehensively
The antioxidant activity index (AAI) was calculated using both concentration of DPPH and IC50 to determine a constant for each antioxidant and shown in the Fig. 4b

Summary

Introduction

Drynaria quercifolia L. (Family- Polypodiaceae) is a fern grows in Bangladesh used in traditional healing by the Garo tribe of Mymensingh district. The Garo is one of them and considered as an ethnic group of ‘Tibbeti Borman,’ belonging to the Mongolian human race They are living in Mymensingh, Tangail, Netrokona, Sylhet and Sunamgonj districts. They have their own curative practices involving many medicinal plants as evident from some ethnobotanical surveys [2, 3]. These plants can be systematically evaluated to explore their healing abilities.

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