Abstract
The study is aimed at establishing an efficient protocol for â€in vitro†medium term conservation of sweet potato. Three medium variants and three different temperatures were used for this purpose. In this experiment 6 varieties of sweet potato were tested (Juhwangmi, KSC1, Yulmi, KSP1, Hayanmi and VSP1). Uninodal segments of fully developed sweet potato plants already established â€in vitro†were cultured on MS basal medium with and without osmotic regulators (mannitol and sorbitol). The cultures were incubated at three different temperatures: 24±2ºC, 20±2ºC and 16±2ºC. After 140 days of â€in vitro†conservation shoots height, number of leaves, number of roots and survival rate (%) were measured. Culture condition variants V1 (MS, 24±2ºC) and V2 (MS + 10g/l mannitol + 10 g/l sorbitol, 20±2ºC) ensured the best results regarding shoots height, number of leaves and number of roots. The highest survival rate (87.78% and 84.44%, respectively) were observed in the V2 and V3 (MS + 15g/l mannitol + 15 g/l sorbitol, 16±2ºC) culture condition variants. The slow growth technique allowed the successful â€in vitro†medium term conservation of sweet potato genotypes.
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