In Vitro Investigation of Trichoderma Strain Potential Against Fusarium Wilt of Tomato

Wiam Benlamoudi,Wassima Lakhdari,Omar Guezoul,Abderrahmene Dehliz

doi:10.33794/qjas.2021.168288

Abstract

Three modes of action of Trichoderma strain are evaluated, with in vitro tests, in order to verify the potential of this antagonistic against Fusarium oxysporum f. sp. lycopersici isolated from tomato plant in Oued Righ region, Algerian southeast. Inhibition rates of Fusarium wilt of the order of 56, 65 and 70% are respectively obtained with antibiosis, competition and mycoparasitism mechanisms. Results, analyzed by ANOVA, are confirmed that the biological agent showed significant fungistatic effect towards Fusarium wilt of tomato most importantly by mycoparasitism that constitutes the most effective mechanism among all the tests applied.

Highlights

In Oued Righ region controlling of Fusarium diseases by using chemicals is the first and the only solution the farmer looks for, due to the aggressiveness of this fungus and the lack of biological alternatives
Trichoderma spp. are worldwide known for their antifungal inhibitor power against a large range of soil born pathogenic fungi [1,2,3,4]. This potency is expressed by several mechanisms involved in antagonistic interactions like competition, antibiosis and mycoparasitism
Screening of biocontrol agents needs to monitor secondary metabolites and enzymes [5], because they play the same function with chemical pesticides

Summary

Introduction

In Oued Righ region (southeastern of Algeria) controlling of Fusarium diseases by using chemicals is the first and the only solution the farmer looks for, due to the aggressiveness of this fungus and the lack of biological alternatives. Under certain conditions Fusarium diseases can be controlled by using natural performant antagonistic. Trichoderma spp. are worldwide known for their antifungal inhibitor power against a large range of soil born pathogenic fungi [1,2,3,4]. This potency is expressed by several mechanisms involved in antagonistic interactions like competition, antibiosis and mycoparasitism. Chitinase, oxidase, protease and glucanase are enzymes associated with fungal cell wall lysis that are produced by Trichoderma species in order to increase plant defence against the pathogen infection [9,10]

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Conclusion