In vitro investigation effects of 4-Hydroxyacetophenone on rat thoracic aorta's vasomotor activity

Abstract

Background: Z. clinopodioides Lam. is also known as a ''Field Mint'' Seven compounds which have vasodilator activity have been isolated from Z. clinopodioides Lam. and two of them are phenolics compounds and these are acetovanillone, 4-HAP, four of them are flavonoids and these are acacetin, apigenin, chrysin, thymonin, one derivat of cinnamic acid and ethyl 4-coumarate. Objective: In this study, it was aimed that was defined vasodilator activiy mechanisms of fenolic compound 4-HAP on isolated rat thorasic aorta. Material and Method: In this study 40 male adult Sprague Dawley rats were used. Prepared rings were laid out into the 20 ml organ bath with Krebs solution. Rings were stretched by 1g and they were subjected to 1 hour incubation period. In the end of the incubation period, PE, KCI, nifedipine, L-NAME, 4-HAP, SQ22.536, ODQ, ACh, SKF96365, Propranolol, Atropin, TEA, Gibenclamide, 4-aminopyridine and U73122 were implemented to bath with a protocol. Results: Mechanisms of relaxed effect the of 4-HAP were assigned by using antagonists. It was observed that vasorelaksan effect of 4-HAP on endothelilal aorta smooth muscle contractions which had been inductioned by PE under the existance of L-NAME was considerably inhibited. Conclusion: It was stated that 4-HAP relaxed PE and KCI contractions and owing to this activity endothel intact tissues on L-NAME existance notably decreased because of NO pathyway. It is firmly believed that as relaxed effect of 4-HAP declines remarkably under the existance of 4-aminopyridine and nifedipine on endothel denuded aorta rings, activity could be on K+ channel and L-type Ca+2 channel. Abbreviations Used: 4-HAP: 4-hydroxyacetophenone, SQ22536: 9-(Tetrahydro-2-furanyl)-9H-purin-6-amine, 9-THF-Ade, L-NAME: N (omega)-nitro-L-arginine methyl ester, DMSO: Dimethyl sulfoxide, 4-AP: 4-aminopyridine, TEA: Tetraethylammonium, ODQ: 1H-(1,2,4) oxadiazolo(4,3-a)quinoxalin-1-one, SKF96365: 1-[β-(3-(4-Methoxyphenyl)propoxy)-4-methoxyphenethyl] 1H-imidazolehydrochloride, 1-[2-(4-Methoxyphenyl)-2-[3-propoxy]ethyl]imidazole, U73122: 1-[6-[((17β)-3-Methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione, ACh: Acetylcholine, KCI: Potassium chloride, IP3: Inositol triphosphate, DAG: Diacylglycerol.