Abstract
Problem statement: The role of 5-HT has been investigated in many behavioral activities. Thus, studies using raphe lesion showed that 5-HT is involved in sleep, general activity levels, habituation, aggression, pain sensitivity and morphine analgesia, avoidance behavior, self-stimulation and water consumption. Approach: The metabolic interaction between serotonin (5- hydroxytrptamine) and indole-3-aldehyde and xanthine via aldehyde oxidase (EC 1.2.3.1) and xanthine oxidase (EC 1.1.3.22), respectively, were studied in liver tissue homogenate of Dunkin-Hartley guinea pigs by following the decrease in substrate concentration using spectrophotometer. Homogenates of liver were incubated with indole-3-aldehyde in the presence and absence of serotonin or (chlorpromazine and allopurinol a potent and selective inhibitors for aldehyde oxidase and xanthine oxidase, respectively). Oxidation of indole-3-aldehyde to indole-3-acetic acid was reduced up to 63.2% in the presence of serotonin (100 µM), while oxidation of xanthine to uric acid was reduced up to 51.6% under the same conditions. Results: In comparison, incubation of the substrates with their specific inhibitors (100 µM of chlorpromazine and 100 µM allopurinol) give almost complete inhibition. These results demonstrate that in the guinea pig liver a metabolic interaction between serotonin and indole-3-aldehyde or xanthine via molybdenum hydroxylases system may take place in liver, which is the main tissue for xenobiotics detoxification. Conclusion: The overall conclusion from this research is that serotonin could be a protector for neurons and other tissue from the insult of oxidation of aldehydes and xanthines by molybdenum hydroxylases.
Highlights
Molybdenum-enzymes are needed for key aldehyde), thereby converting them to the respective carboxylic acid
Only two classes neurons of the brain steam has attracted the attention of are present in human and other mammals, namely, anatomists since the time of Ramony Cajal, who xanthine oxidase family which represented by xanthine described these cells as large multipolar neurons with dehydrogenase, aldehyde oxidase, pyridoxal oxidase uncertain projections
Investigators had general, reactions catalyzed by molybdenum-enzymes known of a blood-brone chemical that produced are characterized by the transfer of an oxygen atom, vasoconstrictions (a’ Serum’ factor that affected blood derived from or incorporated into water, to vessel ‘tonus’ the same Serotonin) and of a or from a substrate in a two-electron redox reaction. substance present in the gut that increased intestinal Aldehyde oxidase proteins are cytoplasmic enzymes motility
Summary
Molybdenum-enzymes are needed for key aldehyde), thereby converting them to the respective carboxylic acid (indole-3-acetic acid). Molybdenum hydroxylases, aldehyde oxidase and ascending projections that innervate virtually all areas xanthine oxidase, activity was monitored, at 37°C, using 100 μm indole-3-aldehyde It has been know from diluted 1:40 dilutions) and 50 μM xanthine as long time that serotonin is synthesis and functioning substrates, respectively, in 67 mm Sorenson's phosphate outside the CNS and it had been found in almost all buffer, pH 7.0, containing 100 μm EDTA. The action of 5-HT upon postsynaptic receptors, as studied by iontophoretic application, is inhibitory (Tecott, 2007) This action is terminated by reuptake into the presynaptic terminal; Fig. 1: The effect of varying serotonin concentrations on the reduction of 76 μM potassium ferricyanide by electrons generated during the oxidation of 100 μM indole-3-aldehyde by the uptake system has a high specificity and does not guinea pig liver aldehyde oxidase.
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