In vitro inhabitation of Zika virus with Reduning injection

Abstract

Objective To explore the in vitro inhabitation effect of Reduning injection against Zika virus (ZIKV). Methods The median toxic concentration (TC50) and the maximal nontoxic concentration (TC0) of Reduning injection on the Vero cells was measured. The effects of high, medium and low concentration of Reduning on cytopathic effects and viral load on the vero cells infected with ZIKV were determined by cell viability assay, immunofluorescence and qRT-PCR. The in vitro inhibitory effect of Reduning injection was discussed. Results The TC50 and TC0 of Reduning injection on the Vero cells was 134.3±5.5 mg/mL and 40.5±5.8 mg/mL, respectively. GLO result showed that cell viabilities were (5.04×106±0.81×106), (4.75×106±0.26×106), (5.0×106±0.19×106), (4.8×106±0.03×106), (4.5×106±0.03×106), (3.89×106±0.14×106), (2.6×106±0.27×106), (2.1×106±0.05×106) and (0.3×106±0.18×106) cells/mL in 10.24, 5.12, 2.56, 1.28, 0.32, 0.08, 0.04, 0.02 and 0.00 mg/mL Reduning injection groups, respectively. The differences in cell proliferation among those groups was statistically significant (F=202.60, P<0.01). The microscopic observation indicated that the cells treated with medium concentration of Reduning injection were significantly better than the control group, However, the titers of ZIKV in the cell supernatants treated with high, medium and low concentrations of the Reduning injection showed no statistically significant difference. Conclusions The Reduning injection has obvious protective effect on the cells infected with the zika virus, but there was no in vitro anti-virus effect against Zika virus. Key words: Antiviral agents; Chinese herbal; Treatment Outcome; Zika virus; Reduning injection