In vitro incubation of bovine ovarian follicles: indications for an active and regulated renin-angiotensin system.

Abstract

1. The ovarian follicular renin-angiotensin system was investigated by using an in vitro incubation method, based on tissue incubation of individual bovine follicles. 2. Very high and varying concentrations of active renin (median 18.0 GU/kg, range 2.1-107 GU/kg; n = 101) and prorenin (11.7 GU/kg, range < 2.6-142 GU/kg; n = 101) existed in unincubated ovarian follicular tissue. 3. Active renin and prorenin increased 35 and 959%, respectively, in follicular wall tissue and incubation medium during 72 h of in vitro incubation. The protein synthesis inhibitor cyclohexamide inhibited active renin and prorenin formation. No activation of prorenin occurred in incubation medium or follicular fluid in vitro. Active renin was degraded during incubation. 4. Primarily prorenin but also active renin were secreted into the incubation medium. Secretion was directed both to the internal and external surface of the follicular wall. 5. Follicle-stimulating hormone (FSH) slightly stimulated the synthesis and secretion of prorenin in vitro. 6. The synthesis and secretion rates of active renin and prorenin varied markedly between individual follicles. 7. The finding of very high and varying concentrations of active renin in follicular wall tissues together with the formation and secretion of active renin during in vitro incubation provide further evidence for an active and regulated renin-angiotensin system in ovarian follicles.